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gel doc 2000 imaging system (bio[rad)  (Bio-Rad)


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    Bio-Rad gel doc 2000 imaging system (bio[rad)
    Gel Doc 2000 Imaging System (Bio[Rad), supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel+doc+2000+imaging+system+(bio%5Brad)/gel+doc+1000/med_rxiv__2023__12__23__23300356-283-19-24
    Average 90 stars, based on 1 article reviews
    gel doc 2000 imaging system (bio[rad) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Integrated identification and mechanism exploration of bioactive ingredients from Salvia miltiorrhiza to induce vascular normalization.
    Article Snippet: Background: The clinical management of ischemic disease and cancer is complex, with disruptions in local vascular function and tumor angiogenesis contributing to blood stasis, which complicates treatment strategies.. Salvia miltiorrhiza, a natural product, is known to restore vascular structure and function.. However, its specific roles in concurrently addressing ischemic disease and cancer within the same organism remain poorly

    Article Title: <i>CircFOXO3</i> mediates hypoxia‐induced autophagy of endometrial stromal cells in endometriosis
    Article Snippet: 1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 2Shandong Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China 3Department of Obstetrics and Gynecology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China 4Department of Reproductive Medicine, Wuhan No.1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Then, the resulting amplicon length and the absence of non-specific bands were evaluated by endpoint PCR using an initial quantity of 50 ng of cDNA as a template derived from the HMC3 cell line and the BioMixTM Red (Meridian Bioscience—Bioline), according to the manufacturer's proceedings. .. The amplification protocol was the following: 94 °C for 2 min enabling the first template denaturation; then the amplification steps repeated for 25 cycles (94 °C for 30'', 60 °C for 30'') and a final step at 72 °C per 5 min. PCR products were separated onto 2% ( w/v ) agarose gel electrophoresis, stained with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and finally observed using the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Total RNA quantification was performed using NanoDropTM 1000 spectrophotometer (Thermo Scientific) and RNA quality was assessed at absorbance values of 280/260 nm and 230/260 nm. .. RNA integrity was then evaluated by 1% agarose gel electrophoresis in TAE buffer with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and analyzed with the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: First Report of Marek's Disease Virus in Commercial Turkeys in Slovenia.
    Article Snippet: Marek’s disease (MD), caused by Mardivirus gallidalpha 2 (GaAHV-2), also known as MD virus (MDV), is a lymphoproliferative disease that primarily affects chickens.. Recently, MDV has been detected in lymphomatous tumors in turkeys in various countries.. Between 2021 and 2023, three cases ranging from no to severe clinical disorders (depression, lameness, and increased mortality) occurred in commercial turkey flocks in Slovenia.

    Article Title: Application of pulsed-field gel electrophoresis for molecular identification of pathogenic Leptospira species in Iran: a rapid and reliable method
    Article Snippet: Following electrophoresis, the gels were stained with ethidium bromide (1 μg/mL) from Sigma (USA) and then destained in water for 20 minutes. .. Fragment patterns were analyzed by visualizing the gels on a UV Tran illuminator and capturing photographs using an imaging system (Gel Doc 2000 System, Bio-Rad Laboratories). ..

    Article Title: First Report of Marek’s Disease Virus in Commercial Turkeys in Slovenia
    Article Snippet: PCR products were loaded onto 1.8% agarose gel (Sigma-Aldrich, St. Louis, MO, USA), and electrophoresis was performed at 130 V for 30 min. .. The gel was stained with ethidium bromide, and bands were visualized using the Bio-Rad Gel Doc 2000 Imaging System, on Quantity One 4.4.0 analysis software. .. Bands were excised from the gel and purified using the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Duren, Germany.).

    Expressing:

    Article Title: Integrated identification and mechanism exploration of bioactive ingredients from Salvia miltiorrhiza to induce vascular normalization.
    Article Snippet: Background: The clinical management of ischemic disease and cancer is complex, with disruptions in local vascular function and tumor angiogenesis contributing to blood stasis, which complicates treatment strategies.. Salvia miltiorrhiza, a natural product, is known to restore vascular structure and function.. However, its specific roles in concurrently addressing ischemic disease and cancer within the same organism remain poorly

    Incubation:

    Article Title: <i>CircFOXO3</i> mediates hypoxia‐induced autophagy of endometrial stromal cells in endometriosis
    Article Snippet: 1Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 2Shandong Key Laboratory of Reproductive Medicine, Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China 3Department of Obstetrics and Gynecology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China 4Department of Reproductive Medicine, Wuhan No.1 Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China

    Amplification:

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Then, the resulting amplicon length and the absence of non-specific bands were evaluated by endpoint PCR using an initial quantity of 50 ng of cDNA as a template derived from the HMC3 cell line and the BioMixTM Red (Meridian Bioscience—Bioline), according to the manufacturer's proceedings. .. The amplification protocol was the following: 94 °C for 2 min enabling the first template denaturation; then the amplification steps repeated for 25 cycles (94 °C for 30'', 60 °C for 30'') and a final step at 72 °C per 5 min. PCR products were separated onto 2% ( w/v ) agarose gel electrophoresis, stained with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and finally observed using the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Then, the resulting amplicon length and the absence of non-specific bands were evaluated by endpoint PCR using an initial quantity of 50 ng of cDNA as a template derived from the HMC3 cell line and the BioMixTM Red (Meridian Bioscience—Bioline), according to the manufacturer's proceedings. .. The amplification protocol was the following: 94 °C for 2 min enabling the first template denaturation; then the amplification steps repeated for 25 cycles (94 °C for 30'', 60 °C for 30'') and a final step at 72 °C per 5 min. PCR products were separated onto 2% ( w/v ) agarose gel electrophoresis, stained with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and finally observed using the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Agarose Gel Electrophoresis:

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Then, the resulting amplicon length and the absence of non-specific bands were evaluated by endpoint PCR using an initial quantity of 50 ng of cDNA as a template derived from the HMC3 cell line and the BioMixTM Red (Meridian Bioscience—Bioline), according to the manufacturer's proceedings. .. The amplification protocol was the following: 94 °C for 2 min enabling the first template denaturation; then the amplification steps repeated for 25 cycles (94 °C for 30'', 60 °C for 30'') and a final step at 72 °C per 5 min. PCR products were separated onto 2% ( w/v ) agarose gel electrophoresis, stained with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and finally observed using the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Total RNA quantification was performed using NanoDropTM 1000 spectrophotometer (Thermo Scientific) and RNA quality was assessed at absorbance values of 280/260 nm and 230/260 nm. .. RNA integrity was then evaluated by 1% agarose gel electrophoresis in TAE buffer with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and analyzed with the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Staining:

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Then, the resulting amplicon length and the absence of non-specific bands were evaluated by endpoint PCR using an initial quantity of 50 ng of cDNA as a template derived from the HMC3 cell line and the BioMixTM Red (Meridian Bioscience—Bioline), according to the manufacturer's proceedings. .. The amplification protocol was the following: 94 °C for 2 min enabling the first template denaturation; then the amplification steps repeated for 25 cycles (94 °C for 30'', 60 °C for 30'') and a final step at 72 °C per 5 min. PCR products were separated onto 2% ( w/v ) agarose gel electrophoresis, stained with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and finally observed using the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR
    Article Snippet: Total RNA quantification was performed using NanoDropTM 1000 spectrophotometer (Thermo Scientific) and RNA quality was assessed at absorbance values of 280/260 nm and 230/260 nm. .. RNA integrity was then evaluated by 1% agarose gel electrophoresis in TAE buffer with SYBRTM Safe DNA Gel Stain (Invitrogen Carlsbad, CA, USA) and analyzed with the gel imaging system Gel Doc 2000 (Bio-Rad, Hercules, CA, USA). ..

    Article Title: First Report of Marek's Disease Virus in Commercial Turkeys in Slovenia.
    Article Snippet: Marek’s disease (MD), caused by Mardivirus gallidalpha 2 (GaAHV-2), also known as MD virus (MDV), is a lymphoproliferative disease that primarily affects chickens.. Recently, MDV has been detected in lymphomatous tumors in turkeys in various countries.. Between 2021 and 2023, three cases ranging from no to severe clinical disorders (depression, lameness, and increased mortality) occurred in commercial turkey flocks in Slovenia.

    Article Title: First Report of Marek’s Disease Virus in Commercial Turkeys in Slovenia
    Article Snippet: PCR products were loaded onto 1.8% agarose gel (Sigma-Aldrich, St. Louis, MO, USA), and electrophoresis was performed at 130 V for 30 min. .. The gel was stained with ethidium bromide, and bands were visualized using the Bio-Rad Gel Doc 2000 Imaging System, on Quantity One 4.4.0 analysis software. .. Bands were excised from the gel and purified using the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Duren, Germany.).

    other:

    Article Title: CCDC78: unveiling the function of a novel gene associated to hereditary myopathy
    Article Snippet: Proteins were revealed by Clarity MaxTM Western ECL Substrate (Bio[Rad) according to the manufacturer’s instructions and images acquisition from Gel Doc 2000 Imaging System (Bio[Rad) were performed with Quantity One® 1-D analysis software.

    Software:

    Article Title: First Report of Marek's Disease Virus in Commercial Turkeys in Slovenia.
    Article Snippet: Marek’s disease (MD), caused by Mardivirus gallidalpha 2 (GaAHV-2), also known as MD virus (MDV), is a lymphoproliferative disease that primarily affects chickens.. Recently, MDV has been detected in lymphomatous tumors in turkeys in various countries.. Between 2021 and 2023, three cases ranging from no to severe clinical disorders (depression, lameness, and increased mortality) occurred in commercial turkey flocks in Slovenia.

    Article Title: First Report of Marek’s Disease Virus in Commercial Turkeys in Slovenia
    Article Snippet: PCR products were loaded onto 1.8% agarose gel (Sigma-Aldrich, St. Louis, MO, USA), and electrophoresis was performed at 130 V for 30 min. .. The gel was stained with ethidium bromide, and bands were visualized using the Bio-Rad Gel Doc 2000 Imaging System, on Quantity One 4.4.0 analysis software. .. Bands were excised from the gel and purified using the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Duren, Germany.).



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